Selective Cytotoxicity of Novel Copper (II) Complex on Colon Cancer Cell Lines



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Submitted Aug 10, 2022
Published Oct 22, 2022
10.24018/ejchem.2022.3.3.115

Abstract viewed = 305 times

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The synthesis of a novel copper (II) complex, namely, [Cu(phen)(NO2-impi)]2+ (where phen is 1,10-phenanthroline; NO2-impi is 2-(2-(4-nitrophenyl)-5-(pyridin-2-yl)-1H-imidazol-4-yl) pyridine, ZU-3, was performed. UV titration and viscosity measurements were used to determine its interaction with ds-DNA. The binding constant of complex to ds-DNA was 1.3x105 M-1. The Stern-Volmer extinction constant (KSV) for ZU-3 was calculated as 1.2 x 105 M-1. The complex was screened against five human cell lines, namely A 549, PC-3, BEAS-2B, HCT-116 and MDZMB-231, and it was found that ZU-3 shows selective cytotoxicity on colon cancer cells. It has been found that ZU-3 reduced HCT-116 cell density and caused pyknotic morphology that are associated with cell death by apoptosis.

Keywords: Apoptosis, Copper (II) Complex, Cytotoxicity, DNA Binding, HCT-116 Cell Line.

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