FID-Gas Chromatography of West-Algerian Cucurbita pepo (or Pumpkin) Seed Oil Extract



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Submitted Aug 6, 2021
Published Aug 31, 2021
10.24018/ejchem.2021.2.3.69

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Fatty acid and phytosterol vegetable oils were extracted from seeds of Cucurbita pepo (or pumpkin) into (v/v) hexane. The extract obtained was characterized by the contents of sterols, unsaturated and saturated fatty acids. The content of the bioactive compounds was determined by gas-chromatography FID method. Pumpkin seed oil extracts showed higher content of poly-unsaturated fatty acids (49.10%) than that from saturated fatty acids (28%). Sterols exhibited different molecules dominated by β-sistostérol (47%), Stigmastérol (23.6%) and campesterol (21.5%). Several peaks were present on the FID GC chromatogram of two extracts (fatty acids and sterols respectively). GC spectra confirmed the presence of the predominant bioactive compounds (PUFA, β-sistostérol , Stigmastérol and campesterol). The composition of pumpkin seed oil extract was characterized by FID-GC spectra with maximum at 280 nm.

Keywords: Cucurbita pepo, fatty acid, gas-chromatography, phytosterol, pumpkin

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