Anti-Hyperglycaemic and Anti-Oxidative Effect of Merremia Tridentata (Linn) Hallier F Ethanol Leaf Extract



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Submitted Jan 3, 2022
Published Feb 5, 2022
10.24018/ejchem.2022.3.1.87

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The anti-hyperglycaemic and anti-oxidative effect of Merremia tridentata (Linn) Hallier F ethanol leaf extract (MTELE) was evaluated in this study. Diabetes was induced on male albino wistar rats by a single intra-peritoneal injection of 65mg/kg body weight dose of streptozotocin. The rats were divided into seven groups and treated accordingly with varying doses (50 mg/kg, 75 mg/kg and 100 mg/kg body weight) of MTELE for seven weeks. Body weight and plasma glucose levels were monitored while antioxidant parameters, catalase (CAT), superoxide dismutase (SOD) activities, reduced glutathione (GSH) concentration and levels of thiobarbituric acid reacting substances (TBARS) were determined in the liver and kidney tissues. There was a significant (p<0.05) reduction in the weight of the diabetic control group which was significantly (p<0.05) ameliorated by the treatment. Plasma glucose levels were significantly (p<0.05) reduced by 50.6%, 62.1% and 72.68% in diabetic rats treated with 50 mg/kg, 75 mg/kg and 100mg/kg body weight dose of MTELE respectively. There was a significant (p<0.05) improvement in all the antioxidant parameters investigated in this study in both the liver and kidney tissues at all levels of treatment (increase in the activities of CAT and SOD, increase in tissue content of GSH and drastic reduction of TBARS level). The extract at 100mg/kg body weight dose on normal rats showed a significant (p<0.05) improvement in the status of SOD in the kidney, GSH and TBARS in the liver when compared with the normal control group. MTELE demonstrated a good anti-hyperglycaemia and antioxidant potential and should be further investigated for development of lead compound capable of preventing and treating oxidative stress related complications in diabetics.

Keywords: Anti-hyperglycaemic, Anti-oxidation, Merremia tridentata, Liver and Kidney

References

  1. K. Arunachalam, and T. Parimelazhagan, Antidiabetic activity of aqueous root extract of Merremia tridentata (L.) Hall. f. in streptozotocin–induced–diabetic rats. Asian Pacific Journal of Tropical Medicine, 2012; 5(3): 175-179.
     Google Scholar
  2. F. Giacco and M. Brownlee, Oxidative stress and diabetic complications. Circulation Research, 2010; 107(9):1058-1070.
     Google Scholar
  3. J.W. Baynes and S. R. Thorpe, Role of oxidative stress in diabetic complications: a new perspective on an old paradigm. Diabetes, 1999; 48:1–9.
     Google Scholar
  4. A. Ceriello, Oxidative stress and diabetes-associated complications. Endocrine Practice, 2006; 12(1):60-62.
     Google Scholar
  5. R., Nimenibo-Uadia and A.U., Osagie, Effect of Ficus exasperata (Vahl) Aqueous leaf extract on normal and Alloxan diabetic Rats. Nigerian Journal of Biochemistry and Molecular Biology, 2001; 16 (1): 67-71.
     Google Scholar
  6. S. E. Atawodi. and A. Muazu, Effect of aqueous extract of Psidium guajava on glucose levels in normoglycaemic and alloxan-induced diabetic rats. Nigerian Journal of Biochemistry and Molecular Biology, 2003; 13(2):125-128.
     Google Scholar
  7. M. Jung M, Park H. C. Lee, Y. H. Kang, E. S. Kang and S. K. Kim, Antidiabetic agents from medicinal plants. Curr Med Chem, 2006; 13:1203-1218.
     Google Scholar
  8. S. O. Oyedemi, E. A. Adewusi, O. A, Aiyegoro, D. A. Akinpelu, Antidiabetic and haematological effect of aqueous extract of stem bark of Afzelia africana (Smith) on streptozotocin-induced diabetic Wistar rats. Asian Pac J Trop Biomed, 2011; 1(5):353-358.
     Google Scholar
  9. R. Nimenibo-Uadia, and K. Nwachukwu, Biochemical Evaluation of Harungana Madagascariensis Lam Aqueous Leaf Extract in Diabetic Rats: International Research Journal of Natural Sciences, 2017;5(2):1-11.
     Google Scholar
  10. M. Kamalutheen, S. Gopalakrishnan and T.S. Ismail, Anti-inflammatory and anti-arthritic activities of Merremia tridentata (L.) Hall. F. E-J Chem, 2009; 6: 943-948.
     Google Scholar
  11. O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951; 193: 265–275.
     Google Scholar
  12. H. P. Misra, and I. Fridovich, The role of superoxide anion in the antioxidant of epinephrine and simple assay for SOD. J Biol Chem 1972; 247: 3170-3175.
     Google Scholar
  13. A. K. Sinha, Colorimetric assay of catalase. Anal Biochem 1972; 47: 389-394.
     Google Scholar
  14. U.N. Tripathi and D. Chandra. Anti-hyperglycemic and anti-oxidative effect of aqueous extract of Momordica charantia pulp and Trigonella foenum graecum seed in alloxan-induced diabetic rats. Indian J. Biochem Biophys., 2010; 47:227-233.
     Google Scholar
  15. G.L. Ellman. Tissue sulphydryl groups. Arch Biochem Biophys. 1995; 82:70-77.
     Google Scholar
  16. R. Rajagopalan, A. Kode, S. V. Penumatha, N. R. Kallikat and P. M. Venugopal, Comparative effects of curcumin on alcohol and PUFA induced oxidative stress. J. Pharm Pharmaceut Sci., 2004; 7: 274-283.
     Google Scholar
  17. H. Okhawa, N. Ohishi, and K. Yagi, Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry, 1979; 95(2): 351-358.
     Google Scholar
  18. O. A. Adebiyi, D.A. Ameh, E. Onyike and D. B. James, Acute and chronic toxicity study of ethanol leaf extract of Merremia tridentata (Linn) Hillier F. European Journal of Advanced Chemistry Research 2021;2(3):14-20.
     Google Scholar
  19. X. F. Zhang, and B.K. Tan, Antihyperglyceamic and anti-oxidant properties of Andrographis paniculata in normal and diabetic rats, Clin Exp Pharmacol Physiol, 2000;27:358-363.
     Google Scholar
  20. S. Lenzen, The mechanism of alloxan and streptozotocin-induced Diabetes, Diabetologia, 2008; 51:216-226.
     Google Scholar
  21. J. V. Hunt, R. T. Dean, and S. P. Wolff, Hydroxyl radical production and autoxidative glycosylation. Glucose autoxidation as the cause of protein damage in the experimental glycation model of diabetes mellitus and ageing. Biochem J; 1988; 256(1): 205-212.
     Google Scholar
  22. J. W. Baynes, Role of oxidative stress in development of complications in diabetes. Diabetes, 1991; 40:243-250.
     Google Scholar
  23. R. A. Kowluru, T. S. Kern, and R Engerman, Abnormalities of retinal metabolism in diabetes or experimental galactosemia IV. Antioxidant defense system. Free Radical Biol Med, 1997; 22: 587-592.
     Google Scholar
  24. T.J. Lyons and A. J. Jenkins (1997). Glycation, oxidation and lipoxidation in the development of diabetes: a carbonyl stress hypothesis. Diabetes Rev 1997; 5:365-391.
     Google Scholar
  25. O. P. Upahyay, R.M. Singh, and K. Dutta, Studies on anti-diabetic medicinal plants used in Indian folklore. Argyavaidyan, 1996; 9(3):159-167.
     Google Scholar
  26. S. Banerjee, H..Singh and T. K. Chatterjee Evaluattion and antidiabetic and antihyperlipidemic potential of methanolic extract of Juniperus communis (L) in streptozotocin-nicotinamide induced diabetic rats. Int J Pharma Bio Sci; 2013; 4(3):10-17.
     Google Scholar
  27. K. Ravi, B. Ramachandran and S, Subramanian, Effect of Eugenia jambolana seed kernel on antioxidant defense system in streptozotocin-induced diabetes in rats. Life Sci; 2004;75: 2717-2731.
     Google Scholar
  28. S. Adikay, R. Radha and B. Koganti, Effect of methanolic extract of aerial parts of Merremia tridentata on gentamicin-induced nephrotoxicity in rats, Natural Products: An Indian Journal, 2008;4(2):174-176.
     Google Scholar
  29. K. Sowndhararajan, J. M. Joseph, K. Arunachalam, and S. Manian. Evaluation of Merremia tridentata (L.) Hallier F for in vitro antioxidant activity. Food Sci. Biotechnol 2010:19(3): 663-669.
     Google Scholar


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